APA
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Panzer, J., Hiller, H., Cohrs, C., Almaça, J., Enos, S. J., Beery, M., … Speier, S. (2020). Pancreas tissue slices from organ donors enable in situ analysis of type 1 diabetes pathogenesis. JCI Insight.
Chicago/Turabian
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Panzer, J., H. Hiller, C. Cohrs, Joana Almaça, Stephen J. Enos, M. Beery, S. Cechin, et al. “Pancreas Tissue Slices from Organ Donors Enable in Situ Analysis of Type 1 Diabetes Pathogenesis.” JCI insight (2020).
MLA
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Panzer, J., et al. “Pancreas Tissue Slices from Organ Donors Enable in Situ Analysis of Type 1 Diabetes Pathogenesis.” JCI Insight, 2020.
BibTeX Click to copy
@article{j2020a,
title = {Pancreas tissue slices from organ donors enable in situ analysis of type 1 diabetes pathogenesis.},
year = {2020},
journal = {JCI insight},
author = {Panzer, J. and Hiller, H. and Cohrs, C. and Almaça, Joana and Enos, Stephen J. and Beery, M. and Cechin, S. and Drotar, D. M. and Weitz, Jonathan R. and Santini, Jorge and Huber, Mollie K. and Qadir, Mirza Muhammad Fahd and Pastori, R. and Domínguez-Bendala, J. and Phelps, E. A. and Atkinson, M. and Pugliese, A. and Caicedo, A. and Kusmartseva, I. and Speier, S.}
}
In type 1 diabetes (T1D), autoimmune destruction of pancreatic β cells leads to insulin deficiency and loss of glycemic control. However, knowledge about human pancreas pathophysiology in T1D remains incomplete. To address this limitation, we established a pancreas tissue slice platform of donor organs with and without diabetes, facilitating the first live cell studies of human pancreas in T1D pathogenesis to our knowledge. We show that pancreas tissue slices from organ donors allow thorough assessment of processes critical for disease development, including insulin secretion, β cell physiology, endocrine cell morphology, and immune infiltration within the same donor organ. Using this approach, we compared detailed pathophysiological profiles for 4 pancreata from donors with T1D with 19 nondiabetic control donors. We demonstrate that β cell loss, β cell dysfunction, alterations of β cell physiology, and islet infiltration contributed differently to individual cases of T1D, allowing insight into pathophysiology and heterogeneity of T1D pathogenesis. Thus, our study demonstrates that organ donor pancreas tissue slices represent a promising and potentially novel approach in the search for successful prevention and reversal strategies of T1D.
